Dynamic High Capacity Mustang® Q Membrane Units for Scaleable Anion Exchange Chromatography Purification of Adenoviral Vectors
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چکیده
Adenovirus vectors (rAd) are widely used in gene therapy to deliver genes of interest. It is therefore essential to develop a purification process that clears the adenovirus of undesired contaminants while purifying high titre stock adenovirus. The current standard method of purification uses a CsCl density gradient, which is formed during centrifugation. The limitations of this procedure include the speed of the process, the cost of trying to scale up to pharmaceutically relevant levels and the potential carryover of high molecular weight proteins.
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